Disulfide bridging analysis
Proteome Factory offers a broad range of disulfide analyses adapted to your sample requirements. As the formation of a single disulfide bridge results in a mass difference of -2.0157 Da the existance of disulfides within a polypeptide of up to 20 kDa can be validated by determination of the intact protein mass by high-resolution ESI-MS. To validate single disulfides and correct pairing we offer directed proteolysis under non-reducing conditions in order to generate suitable bridged peptides for mass spectrometric detection and fragmentation.
The price per analysis is dependent to:
- the number of disulfide bridges
- the need of proteolysis (single, multiple)
- verification of predicted disulfides vs analysis of all combinations