An important branch of industrial biotechnology is the production of enzymes in large scale. While the proteins are commonly known, subtle differences in the protein sequences to publicly known databases are common. Frequent analytical questions in White Biotechnology thus deal with the identification and confirmation of proteins with respect to such minor amino acid exchanges.

Proteome Factory offers full sequence validation of such proteins with special regard to such sequence modifications. Multiple proteolytic digests are performed, followed by high resolution nanoHPLC-ESI-MS/MS and database search and (if required) de novo sequencing. Intact mass analysis by HPLC-ESI-MS and Edman sequencing can provide complementary data if needed in a particular project.