Based on the sample complexity and the task different combinations of approaches can be used to detect or characterize phosphorylations.

  • Differential setups of phosphoproteins vs dephosphorylated sample
  • Enrichment of phosphoproteins using MOAC (metal oxide affinity chromatography)
  • Enrichment of phosphopeptides with TiO2 columns
  • Neutral loss triggered MS3 experiments
  • HCD or ECD fragmentation
  • Reporter ion scan (i. e. immonium ion of p-Tyrosine)