The comparative quantitative analysis of complex protein samples can be achieved either with 2DE gel-based proteomics or mass spectrometry-based approaches using isobaric tagging like iTRAQ and TMT. Proteome Factory applies both technologies depending on their individual strengths for a particular project and customer's requirements.
iTRAQ and TMT are chemical labels which are reacted with complex protein samples after proteolysis to peptides. Up to eight differentially labelled samples are pooled, separated in a first dimension by strong cation exchange chromatography and analysed by LCMS for protein identification and quantification on a large scale. Many hundred proteins are identified and quantified routinely.
iTRAQ (isobaric tagging for relative and absolute quantification) is available in 4-plex and 8-plex formats, while TMT (tandem mass tags) is available in 2-plex, 6-plex and (since recently) 10-plex formats.
iTRAQ is a registered trademark of SCIEX, for more information click here
TMT is a registered trademark of Proteome Sciences plc, for more information click here