Based on the sample complexity and the task different combinations of approaches can be used to detect or characterize phosphorylations.
- Differential setups of phosphoproteins vs dephosphorylated sample
- Enrichment of phosphoproteins using MOAC (metal oxide affinity chromatography)
- Enrichment of phosphopeptides with TiO2 columns
- Neutral loss triggered MS3 experiments
- HCD or ECD fragmentation
- Reporter ion scan (ie: immonium ion of p-Tyrosine)